| Autor: |
Liu, Na, Zhao, Ya Jie, Wu, Jia Wen, Wei, Yi Ming, Ren, Ru Chang, Zang, Jie, Zhang, Wen Ting, Zhang, Lin, Shen, Qiang, Zhang, Xian Sheng, Zhao, Xiang Yu |
| Předmět: |
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| Zdroj: |
Molecular Breeding; Aug2020, Vol. 40 Issue 8, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Brassinosteroids (BRs) are a class of plant-specific steroid hormones and play an essential role in plant growth and development. The DWARF4 (DWF4) gene encodes a C-22 hydroxylase, which is a rate-limiting enzyme in BR biosynthesis pathway. Here, the DWF4 (ZmDWF4) in maize (Zea mays L.), the ortholog of Arabidopsis DWF4 (CYP90B1), was transformed into elite inbred Q319 and driven maize ubiquitin promoter. In transgenic lines, the level of the endogenous BR precursor canosterone (CS) was significantly enhanced. ZmDWF4 overexpression greatly improved grain yield per ear from 28.3 to 33.5% in transgenic lines compared with that in non-transgenic Q319 plants. Moreover, ZmDWF4 overexpression significantly enhanced heterosis of combinations by increasing both seed number and seed weight up to 20.4% in transgenic hybrids. Further analysis revealed that ZmDWF4 overexpression significantly improved several agronomic traits and leaf photosynthetic ability in transgenic lines. Finally, RNA-seq analysis suggested that overexpressing ZmDWF4 improved cell growth, cell division, and nutrient assimilation in transgenic kernels. Altogether, our results demonstrate that manipulating BR level by overexpressing ZmDWF4 effectively improves maize agronomic traits, and thus, provides a useful biotechnological strategy for the genetic improvement and further commercially favorable maize breeding. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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