| Autor: |
Yuan Lu, Jue Xi, Yao Zhang, Chenzong Li, Wensu Chen, Xiaoqin Hu, Min Zhang, Fengyun Zhang, Hui Wei, Zhi Li, Zhirong Wang, Lu, Yuan, Xi, Jue, Zhang, Yao, Li, Chenzong, Chen, Wensu, Hu, Xiaoqin, Zhang, Min, Zhang, Fengyun, Wei, Hui |
| Předmět: |
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| Zdroj: |
Archives of Medical Science; 2020, Vol. 16 Issue 5, p1119-1129, 11p |
| Abstrakt: |
Introduction: MicroRNAs (miRNAs) are considered as crucial modulators in myocardial ischemia and reperfusion (I/R) injury. The present study aimed to investigate the expression and biological functions of miR-214-5p via targeting Fas ligand (FASLG) in I/R injury.Material and Methods: Lactate dehydrogenase, casein kinase, malondialdehyde assay, reactive oxygen species (ROS) detection and cell apoptosis analysis measured cell damage and cell apoptosis in H9c2 cells under hypoxia/reperfusion (H/R) treatment. Bioinformatics and dual luciferase reporter assays demonstrated the molecular mechanism of miR-214-5p in cardiac cells. 2,3,5-Triphenyltetrazolium chloride (TTC) staining, hematoxylin-eosin (HE) staining and adenovirus injection were performed in I/R treated mice.Results: The expression of miR-214-5p was decreased in H/R injured H9c2 cells compared with control cells (p < 0.001). Overexpression of miR-214-5p reduced cell damage and apoptosis in H9c2 cells under H/R treatment (p < 0.001). Further study revealed that FASLG was a target of miR-214-5p. Enhanced expression of FASLG attenuated the protective function of miR-214-5p in H9c2 cells subjected to H/R injury (P < 0.001). Moreover, the elevated expression of miR-214-5p by adenovirus injection protected cardiac cells from I/R injury in mice (n = 6/per group).Conclusions: We found that miR-214-5p exerted a protective role in I/R injured cardiac cells by direct targeting FASLG in vitro and in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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