| Autor: |
Feng, Liqiang, Wang, Qian, Shan, Chao, Yang, Chenchen, Feng, Ying, Wu, Jia, Liu, Xiaolin, Zhou, Yiwu, Jiang, Rendi, Hu, Peiyu, Liu, Xinglong, Zhang, Fan, Li, Pingchao, Niu, Xuefeng, Liu, Yichu, Zheng, Xuehua, Luo, Jia, Sun, Jing, Gu, Yingying, Liu, Bo |
| Předmět: |
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| Zdroj: |
Nature Communications; 8/21/2020, Vol. 11 Issue 1, pN.PAG-N.PAG, 1p |
| Abstrakt: |
The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation of a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying a codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody and cell-mediated immune (CMI) responses. Intranasal inoculation elicits both systemic and pulmonary antibody responses but weaker CMI response. At 30 days after a single vaccination with Ad5-S-nb2 either intramuscularly or intranasally, macaques are protected against SARS-CoV-2 challenge. A subsequent challenge reveals that macaques vaccinated with a 10-fold lower vaccine dosage (1 × 1010 viral particles) are also protected, demonstrating the effectiveness of Ad5-S-nb2 and the possibility of offering more vaccine dosages within a shorter timeframe. Thus, Ad5-S-nb2 is a promising candidate vaccine and warrants further clinical evaluation. A vaccine protecting from SARS-CoV-2 infection is needed. Here the authors generate a replication-incompetent adenovirus based vaccine expressing SARS-CoV-2 spike, show protection from infection in non-human primates, and analyze the immune response after intramuscular and intranasal vaccination. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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