| Autor: |
Kumar, Kaushal, Singh, K. K., Gupta, M. K., Singh, G. D. |
| Předmět: |
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| Zdroj: |
Journal of Experimental Zoology India; Jul2020, Vol. 23 Issue 2, p1729-1735, 7p |
| Abstrakt: |
Mammary tumours have a frequent tendency to develop the ability of invasiveness, recurrence and metastasis. Assessment of cellular proliferation can provide useful information about mechanism of invasion and can be used in conjunction with pathomorphological parameters like tumour size, nodularity and total cell counts and survival period of the cancer patient as a prognostic indicator. On the basis of invasiveness two distinct broad types of mammary tumours have been well known which are non invasivein situ adenocarcinoma and invasive adenocarcinoma. The mean argyrophilicnucleolarorganising regions (mAgNOR) is the only parameter on histological section to assess both proliferative fraction and speed. Ability of AgNOR staining to assess the ability to assess invasive behavior of tumours does not appear to be evaluated. The study aims at assessment of correlation between them and AgNOR count in NMU induced in situ as well as invasive mammary carcinoma in wistar rats and to further correlate mAgNOR with pathomorphological parameters with an aim to throw more insight on mechanism of tumour invasion. Tumour induction was done by N -nitroso-N-methylurea (NMU) at the dose rate of 50 mg/kg body weight. 1% (W/V) NMU solution in dimethyl sulfoxide (DMSO) was prepared and injected intraperitoneally, once per week for four weeks. Seven out of twelve mammary tumours were histopathologically confirmed for ductular adenocarcinoma in situ while five tumours were low grade invasive ductular adenocarcinoma The t-test showed significantly higher mean values of mAgNOR count, mitotic figure count and different pathomorphological parameters of biological behavior i.e., tumour volume, multinodularity and total cell count in Grade-II than Grade-I tumour of mammary ductular adenocarcinoma indicating their higher values in biologically more aggressive NMU induced tumour in rats. The present study describes a positive correlation between mAgNORs and pathomorphological parameters of biological behavior of NMU induced mammary tumours in rat and also aids in the prognostication of invasive mammary tumours. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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