| Autor: |
Longkumer, Sentiyanger, Jamir, Ajungla, Pankaj, Pranay Punj |
| Předmět: |
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| Zdroj: |
Journal of Experimental Zoology India; Jul2020, Vol. 23 Issue 2, p1305-1310, 6p |
| Abstrakt: |
Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia and alterations in carbohydrate, fat and protein metabolism. Zebrafish (Danio rerio) exhibits many features of vertebrate models apart from physiological and anatomical characteristics of higher organisms that prompted scientists around the world to use it in biomedical research. In this study, we performed a validation study for the development of optimal hyperglycemia in zebrafish involving diabetogenic agents such as alloxan monohydrate (AM), streptozocin (STZ) and glucose water solution (GLU-W). Exposure of 100 mg of AM/dL and 200 mg of AM/dL for 30 mins were not capable of inducing DM in the subjects, whereas exposure of 300 mg of AM/dL and 400 mg of AM/dL for 30 mins were able to induce DM in the zebrafish. Mortality had also been recorded in the group treated with 400 mg of AM/dL. Overall, a dose of 300 mg of AM/dL for 30 mins was found to be suitable to induce DM in the subjects. Zebrafish showed diabetic when treated with AM then after exposure with 1% GLU-W minimum for 30 mins, but when the subjects left normal after induction, blood glucose level went down to time. It was also observed that when the subjects kept in different GLU-W (1%, 2% and 3%) for a minimum of 21 days then it took 21 days to induce DM in the subject treated with 1% GLU-W treated subjects whereas exposure with 1% and 2% GLU-W showed diabetes just after 7 days and 4 days onwards. Streptozocin (0.35 mg/gm of body weight) exposed with its booster doses was found to maintain diabetes in the subject for 21 days without any mortality which may be helpful for the researchers’ working on secondary complications of DM taking zebrafish as a model. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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