| Autor: |
Latypova, Larisa, Puzenko, Alexander, Levy, Evgeniya, Feldman, Yuri |
| Předmět: |
|
| Zdroj: |
Journal of Chemical Physics; 7/28/2020, Vol. 153 Issue 4, p1-8, 8p, 1 Graph |
| Abstrakt: |
In this paper, the fifth of our series focused on the dielectric spectrum symmetrical broadening of water, we consider the solutions of methemoglobin (MetHb) in pure water and in phosphate-buffered saline (PBS). The universal character of the Cole–Cole dielectric response, which reflects the interaction of water dipoles with solute molecules, was described in Paper I [E. Levy et al., J. Chem. Phys. 136, 114502 (2012)]. It enables the interpretation of the dielectric data of MetHb solutions in a unified manner using the previously developed 3D trajectory method driven by the protein concentration. It was shown that protein hydration is determined by the interaction of water dipoles with the charges and dipoles located on the rough surfaces of the protein macromolecules. In the case of the buffered solution, the transition from a dipole-charged to a dipole–dipole interaction with the protein concentration is observed {see Paper III [A. Puzenko et al., J. Chem. Phys. 137, 194502 (2012)]}. A new approach is proposed for evaluating the amount of hydration water molecules bounded to the macromolecule that takes into account the number of positive and negative charges on the protein's surface. In the case of the MetHb solution in PBS, the hydration of the solvent ions and their interaction with charges on the protein's surface are also taken into consideration. The difference in hydration between the two solutions of MetHb is discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
