Next-generation sequencing technology in the diagnosis of five patients with human herpesvirus encephalitis/meningitis.

Autor: JIN Ke, WANG Xiao-juan, GUAN Hong-zhi, QIN Ling-zhi, JIA Ya-zhen, ZHOU Ke-ke, MA Hai-chang, WANG Yue, WANG Fang, XING Li, LI Wei
Předmět:
Zdroj: Chinese Journal of Contemporary Neurology & Neurosurgery; Jul2020, Vol. 20 Issue 7, p638-642, 5p
Abstrakt: Objective To evaluate the value of next-generation sequencing (NGS) of cerebrospinal fluid (CSF) in the diagnosis of human herpesvirus encephalitis/meningitis. Methods The clinical features, laboratory and imaging findings of 5 patients with highly suspected human herpesvirus encephalitis/ meningitis from January to September 2018 were retrospectively analyzed, and then the etiological sources were comprehensively analyzed combined with the results of NGS. Results All of the 5 patients had an acute onset, fever, headache and neck ankylosis. The CSF tests revealed a slight increased level of white blood cell count (74-260) x 106/L. Among them, a total of 4-5406 nucleic acid sequences of human herpesvirus, including human herpesvirus type I (n = 1), human herpesvirus type III (n = 3) and human herpesvirus type IV (n = 1) were detected by NGS, with the coverage varing from 0.08% to 81.01% . Conclusions NGS of CSF can assist the diagnosis of human herpesvirus encephalitis/meningitis, as well as viruses accurate classification, which is worth popularizing and applying in clinic. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index