| Autor: |
De Jesús-Martínez, X., Olmedo-Juárez, A., Rojas Hernández, S., Zamilpa, A., de Gives, P. Mendoza, Lopez-Arellano, M. E., Villa-Mancera, A., Camacho-Díaz, L. M., Cipriano Salazar, M., Olivares-Pérez, J. |
| Předmět: |
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| Zdroj: |
Agroforestry Systems; Aug2020, Vol. 94 Issue 4, p1315-1321, 7p |
| Abstrakt: |
The aim of this study was to evaluate the in vitro lethal effect of a hydroalcoholic extract (HAE) from C. coriaria fruit against eggs and larvae of H. contortus of domestic ruminants. The HAE was assessed using five concentrations: 50, 25, 12.5, 6.15 and 3.12 mg/mL to eggs and 100, 125, 175, 150 and 200 mg/mL to larvae, respectively; 0.5% ivermectin was used as positive control and 4% methanol in distilled water as negative control. The data of larvicidal and ovicidal effect were analysed with a completely randomised design by ANOVA analysis using the general linear model, while lethal concentrations (LC50 and LC90) were estimated by a Probit analysis of the SAS programme. A clear HAE-increased concentration dependence effect was observed on eggs and larvae. The highest activity of the HAE was obtained at the highest concentration (P < 0.05) to obtain a similar effect as the positive control (ivermectin), with LC50 = 22.93 and 10.3 mg/mL and LC90 = 44.0 and 84.18 mg/mL, respectively, for larvae and eggs. The results indicate that the HAE of C. coriaria fruit possesses in vitro ovicidal and larvicidal properties (total phenols methyl gallate) against H. contortus, and it becomes an alternative for in vivo research for the control of gastroenteric nematodes in ruminants, either whole fruits or extracts administered orally. In this context, in vivo studies with this legume are thus necessary. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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