| Autor: |
Khristichenko, Yu., Hushpulian, D. M., Smirnova, N. A., Zakhariants, A. A., Chubar, T. A., Tishkov, V. I., Gazaryan, I. G., Poloznikov, A. A. |
| Zdroj: |
Moscow University Chemistry Bulletin; May2020, Vol. 75 Issue 3, p172-178, 7p |
| Abstrakt: |
Reporter vectors expressing of amino acid residues 16–96 and 16–85 from Neh2-domain of Nrf2 transcription factor fused to firefly luciferase have been constructed. Cell lines stably expressing the above reporter constructs as well as an earlier developed Neh2-luc fusion reporter have been generated using HeLa cells. Comparative analysis of all three reporter lines has been performed using a well-known Nrf2 activator, nordihydroguaiaretic acid. The reporter expressing the minimal fragment of Neh2-domain containing 16–85 aa sequence exhibits a 2-fold higher amplitude of activation compared to Neh2-luc reporter, and thus can be recommended for high throughput screening for Nrf2 activators. A likely reason for the improved response of the above reporter could be ubiquitination of N-terminal lysine residues (7, 10, 11) of luciferase protein adjacent to the ubiquitinated Neh2 sequence. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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