| Autor: |
Chakrabarti, Swarup K., Ghosh, Sanghamitra, Kalapahar, Shovan D., Bose, Gunja, Wasim, Saeeda, Das, Tushar K., Chattopadhyay, Ratna, Chakravarty, Baidyanath |
| Předmět: |
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| Zdroj: |
Journal of Stem Cells; 2019, Vol. 14 Issue 3, p141-151, 11p |
| Abstrakt: |
In Vitro Fertilization (IVF) has been quite effective in the management of infertility till date. Though IVF has been useful, its success rate is still far from satisfactory. One of the key factors that limits IVF success rate is recurrent implantation failure leading to multiple miscarriages. A healthy receptive endometrium is key to successful implantation of good quality embryos and thus can significantly improve IVF success rate. The major challenge of IVF is to increase the chances of embryo(s) implantation in IVF-frozen embryo transfer (IVF-FET) protocol that will reduce the number of repeated cycles of IVF treatment and thereby reducing the possible risks in patients exposed to high dose of gonadotropins used in IVF treatment. Adequate endometrial vascularity and endometrial thickness are key to successful live birth in IVF. However, in spite of the diversity of treatment modalities, the current medical management results modest improvement of inadequate endometrial thickness (ET), vascularity, and subsequent pregnancy rate. Autologous cell therapy is a new concept for improving endometrial function. Peripheral blood mononuclear cells (PBMCs) are the source different type of stem cells having potential to divulge into cell of different lineages. Thus the multidifferentiation potential of PBMCs suggests that they may be a key source of many functional cell types for regenerative medicine. PMBCs are capable of secreting biologically active paracrine factors that induce regeneration by activating the endogenous stem cells present in tissues. In the present study, we investigated the safety and efficacy of intrauterine administration of PBMCs in the proliferative phase of endometrial development prior to embryo transfer in patients in frozen embryo transfer (FET) cycle with inadequate endometrium and/or inadequate vascularity leading to failed implantations and recurrent miscarriages. In the present study, we were able to show that PBMCs isolated from patients' own blood when infused into the uterine cavity in the proliferative phase of the endometrium development prior to FET, significantly improved overall IVF success rate (56.25%) in patients who had minimum of three previously failed FET attempts. This increased success rate was due to significant increase of both ET and vasculartity in infertile patients treated with PBMC Thus, PBMCs isolated from patients' blood can be used effectively to improve the IVF success rate in infertile women who had multiple previous failed FET attempts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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