| Autor: |
JING NING, YU CHANG, RUXIA WANG, ZHIWEI LAN, QING RU, MINGCHUN LIU, CHUNLIAN TIAN |
| Předmět: |
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| Zdroj: |
Acta Poloniae Pharmaceutica; Mar/Apr2020, Vol. 77 Issue 2, p313-317, 5p |
| Abstrakt: |
Diosgenin is a steroidal sapogenin compound, and possesses multiple biological activities including anti-inflammatory, anticancer, immunological regulation, and anti-aging. The current study focused on its antiinflammatory activities and cytotoxicity by analysis of NO production, phagocytosis activity, secretion of TNFa and IL-6 and cell viability in LPS-induced RAW 264.7 cells. An IC50 value of diosgenin of 2.8 pM was calculated for diosgenin by regression of cell viability from concentrations ranging from 0.01 to 25 pM; this indicated that 0.01, 0.02, 0.04 pM diosgenincould reduce phagocytic activity very significantly (p < 0.01) in a dosedependent manner with no cytotoxic effect on the LPS-induced RAW 264.7 cells. However, there was no significant effect on NO content and secretion of TNF-a and IL-6 after diosgenin treatment. The research revealed that low concentrations diosgenin can directly inhibit cell phagocytosis, with no effect on the release of inflammatory mediators and cytokines. This lays a foundation for screening for a safe dose in research and development of derivatives and a new formulation of diosgenin for its anti-inflammatory effect. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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