| Abstrakt: |
To evaluate the protective role of ascorbic acid compared with d-l-á-tocopheryl acetate -sodium selenite combination on toxicity induced by sodium nitrate. Forty two healthy adult male mice divided equally into 6 groups and administered the following daily for 2 weeks : T1, received d-l-α-tocopheryl acetate(20 IU)-sodium selenite (260 mcg) solution 0.5 ml /L (DW) daily. T2, received ascorbic acid solution 0.5 gm/L (DW) daily. T3, received NaNO3 solution 0.5gm/L (DW). T4, received NaNo3 solution orally in dose 170 mg/kg and d-l-α-tocopheryl acetate (20 IU)-sodium selenite (260 mcg) solution; 0.5 ml/L (DW) daily. T5 received NaNO3 solution orally in dose 170 mg/kg and ascorbic acid solution 0.5 gm/L (DW) daily. T6 (Control) received DW daily. Considerable distinction in total WBCs and monocytes counts reported between all groups compared with control. Considerable distinction in total WBCs count reported between T2, T5 and T3 (p<.0001). Considerable distinction in total lymphocytes count between T6 and T2 (p<0.007), T6 and T5 (p<0.044). Considerable distinction in total monocytes count between T3 and T1 (p<0.008); T3 and T2 (p<0.007). Considerable distinction in CRP level between T6 and T1 (p value = 0.006), T3 (p value = 2.54E-06). Considerable distinction in CRP level between T2; T4; T5 and T3 (p<0.0001). Considerable distinction in serum globulin level between T6 and T1, T2, T4, T5. Considerable distinction in serum globulin level between T3 and T1, T2, T4, T5. Considerable distinction in serum cortisol level between T3 and T2, T5 (p = <.0001). NBT reduction was high among T2 (28.8%) and T1 (26.2%). The ability of monocyte–macrophage for NBT reduction was seriously affected in T3 (18.25%). Considerable distinction in intensity of NBT reduction, moderate (p value = 0.053181) as well as marked intensity (p value = 0.003338) among treated groups. D-l-α-tocopheryl acetate-sodium selenite and ascorbic acid have physiological protective effects, immunomodulatory activities and meliorate the hematological and immunotoxic effects of sodium nitrate. [ABSTRACT FROM AUTHOR] |
