| Abstrakt: |
This study was conducted to investigate the effect of adding aqueous extracts of Olea europaea (AEOEL), Rosmarinus officinalis leaves (AEROL), calcium chloride (CaCl2) and their combinations to Tris extender on post-cryopreserved sperm’s cell individual motility and live sperm percentage for low-semen quality of Holstein bulls for different preservation periods (cooling at 50C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). The experiment was carried out using five Holstein bulls of 2.5-3 years old. Pooled semen was equally divided into six groups within one experiment. AEROL (0.04 mg / 25 ml; T2), AEOEL (0.01 mg / 25 ml; T3), CaCl2 (0.02 M; T4), AEROL (0.04 mg/25 ml) + CaCl2 (T5) and AEOEL (0.01 mg / 25 ml) + CaCl2 (T6) were added to Tris extender and comparisons in response were made with the control group (Tris extender, T1). The productive revenue percentage of the aqueous extracts of Olea europaea leaves was 6.6%, the total phenolic compounds of the extract was 28.9 mg GAE / g extract and the total flavonoid compounds was 16.4 mg Rutin/g extract. On the other hand, the productive revenue percentage of the aqueous extracts of Rosmarinus officinalis leaves was 7.2%, the total phenolic compounds of the extract was 73.2 mg GAE / g extract and the total flavonoid compounds was 39.5 mg Rutin/g extract. The differences in sperm’s cell individual motility percentage (except for the superiority of T2 than T6, 1 month PC), live sperm percentage (except for the superiority of T1 than T5, 3 month PC) lacked significance. In conclusion, the AEOEL and AEROL as alone or in combinations with CaCl2 did not significantly affected sperm’s cell individual motility and live sperm percentage for low-semen quality of Holstein bulls. [ABSTRACT FROM AUTHOR] |
