| Autor: |
FALLER, ERWIN MARTINEZ, MOHAMMAD GHAZI, MAIZATUL AMALIA BINTI, ZAJMI, ASDREN |
| Předmět: |
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| Zdroj: |
Journal of Science & Arts; Jun2020, Vol. 20 Issue 2, p425-436, 12p |
| Abstrakt: |
This study aims to evaluate the chitosan-loaded bacteriophage gel (CLBG) for the treatment of tropical skin ulcer (TSU) against Fusobacterium ulcerans. The sewage was collected, isolated and suspended to obtain the phage. The plaque formed from phage propagation was then collected and recorded. The chitosan gel was prepared and mixed with activated bacteriophage lysate and tested using the Minimum Inhibitory Concentration (MIC) and disc diffusion tests. Physico-chemical evaluation of the loaded gel was observed including viscosity, texture, TEM and pH. The plaque-forming units (PFU) of the bacteriophage were 6.8x104 PFU/mL, 2.3x104 PFU/mL, 1.1x104 PFU/mL and 4.0x104 PFU/mL. Physical evaluation revealed a milky yellowish formation of a gel texture with a pH of 4.63. Microscopic evaluation showed the morphology of chitosan and live tailless bacteriophage. The MIC values of CLBG against the selected pathogens were 1.0 x 10-5mL/mL and 1.0 x 10-6 mL/mL, respectively. The zone of inhibition (ZI) for the CLBG was greater (>48mm) compared to gentamicin (positive control >25mm). Statistical analysis using One-Way Anova showed the highly significant value of this study with p<0.001. The CLBG demonstrated a greater synergism effect against the selected pathogen. Hence, the CLBG has a great potential to be a novel drug delivery for the treatment of tropical skin ulcer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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