| Autor: |
Laguette, Mary‐Jessica N., Barrow, Kelly, Firfirey, Firzana, Dlamini, Senanile, Saunders, Colleen J., Dandara, Collet, Gamieldien, Junaid, Collins, Malcolm, September, Alison V. |
| Předmět: |
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| Zdroj: |
Journal of Orthopaedic Research; Aug2020, Vol. 38 Issue 8, p1856-1865, 10p |
| Abstrakt: |
Variants within genes encoding structural and regulatory elements of ligaments have been associated with musculoskeletal soft tissue injury risk. The role of intron 4‐exon 5 variants within the α1 chain of type V collagen (COL5A1) gene and genes of the transforming growth factor‐β (TGF‐β) family, TGFBR3 and TGFBI, was investigated on the risk of anterior cruciate ligament (ACL) ruptures. A case‐control genetic association study was performed on 210 control (CON) and 249 participants with surgically diagnosed ruptures (ACL), of which 147 reported a noncontact mechanism of injury (NON). Whole‐exome sequencing data were used to prioritize variants of potential functional relevance. Genotyping for COL5A1 (rs3922912 G>A, rs4841926 C>T, and rs3124299 C>T), TGFBR3 (rs1805113 G>A and rs1805117 T>C), and TGFBI (rs1442 G>C) was performed using Taqman SNP genotyping assays. Significant overrepresentation of the G allele of TGFBR3 rs1805113 was observed in CON vs ACL (P =.014) and NON groups (P =.021). Similar results were obtained in a female with the G allele (CON vs ACL: P =.029; CON vs NON: P =.016). The TGFBI rs1442 CC genotype was overrepresented in the female ACL vs CON (P =.013). Associations of inferred allele combinations were observed in line with the above results. COL5A1 intron 4‐exon 5 genomic interval was not associated with the risk of ACL ruptures. Instead, this novel study is the first to use this approach to identify variants within the TGF‐β signaling pathway to be implicated in the risk of ACL ruptures. A genetic susceptibility interval was identified to be explored in the context of extracellular matrix remodeling. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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