| Autor: |
Sabriu-Haxhijaha, Arita, Popovska, Olga, Mustafa, Zana |
| Předmět: |
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| Zdroj: |
Journal of Hygienic Engineering & Design; 2020, Vol. 31, p152-156, 5p |
| Abstrakt: |
The seeds and oil of Nigella sativa L. (black cumin) are used in food and pharmacology industry possessing antioxidative, antimicrobial, antihypertensive and antidiabetic properties. The aim of this work was to introduce a simple, inexpensive method for determination of the main compounds in essential oil of black cumin. The isolation of the essential oil of Nigella sativa L. seeds was done with steam distillation followed by extraction with diethyl ether (3 x 10 mL) and dried over sodium sulphate. The identification of the main compounds was performed with thin-layer chromatography (TLC). For chromatographic determination, 2.5 µL of sample standard solutions were spotted on 20 x 20 cm Merck pre-coated TLC plates (60 F254, 250 µm). A comparison study with two mobile phase solvent systems were applied to the analysis, (A) consisted of benzene : glacial acetic acid (1 : 1) and (B) consisted of carbon tetrachloride/acetone/glacial acetic acid (15.2 : 3 : 1). The retention factor (Rf ) less than 0.3 obtained with the (A) mobile phase was not satisfying in the separation of the compounds from the mixture. The identification of one spot-one compound was achieved with (B) mobile phase where the Rf values for two main compounds thymoquinone and dithymoquinone were 0.6 and 0.5, respectively. The high quality of essential oil from black cumin is as a result of the presence of thymoquinone in a quantity > 50%. The oil with nutritive value and pungent bitter taste can be used as a food flavoring additive and as a functional food. The health-promoting bioactive compounds present in Nigella sativa L. essential oil could be detected with TLC as easy-to handle and rapid method for screening of oil quality. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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