| Autor: |
Xia, Xichao, Guan, Cuiui, Chen, Jiawei, Qiu, Maolin, Qi, Jinxu, Wei, Mengwei, Wang, Xiaowei, Zhang, Ke, Lu, Suxiang, Zhang, Linguo, Hua, Chunxiu, Xue, Shipeng, Yao, Lunguang |
| Předmět: |
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| Zdroj: |
Innate Immunity; Jul2020, Vol. 26 Issue 5, p381-397, 17p |
| Abstrakt: |
Sox2 is an embryonal stem cell Ag essential for early embryonic development, tissue homeostasis and immune regulation. In the current study, one complete Sox2 cDNA sequence was cloned from freshwater bivalve Anodonta woodiana and named AwSox2. Histological changes of testis derived from Bisphenol A (BPA) treatment were analyzed by hematoxylin and eosin staining. Expressions of AwSox2 derived from BPA, LPS and polyinosinic:polycytidylic (Poly I:C) challenge were measured by quantitative real-time PCR. The full-length cDNA of AwSox2 contained an open reading frame of 927 nucleotides bearing the typical structural features of Sox2 family. Obvious degeneration, irregular arrangement of spermatids, and clotted dead and intertwined spermatids were observed in BPA-treated groups. Administration of BPA could result in a dose-dependent up-regulation of AwSox2 expression in the male gonadal tissue of A. woodiana. In addition, expression of AwSox2 was significantly induced by LPS and Poly I:C treatment in the hepatopancreas, gill and hemocytes, compared with that of control group. These results indicated that up-regulations of AwSOx2 are closely related to apoptosis of spermatogonial stem cells derived from BPA treatment as well as enhancement of immune defense against LPS and Poly I:C challenge in A. woodiana. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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