| Autor: |
Elshabrawy, Mona A., Abouelhag, Hussien A., Khairy, Eman A., Marie, Hanan Sh., Hakim, Ashraf S. |
| Předmět: |
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| Zdroj: |
Jordan Journal of Biological Sciences; 2020, Vol. 13 Issue 2, p139-144, 6p |
| Abstrakt: |
Coagulase positive staphylococci particularly Staphylococcus aureus are responsible for a variety of illnesses in humans and animals and commonly identified and discriminated by certain virulence factor coagulase (coa) genes. A total of 85 nasal discharge and wound samples were collected from 55 dogs and 30 cats. The samples were cultured and examined for detection of Staphylococcus aureus and then checked using PCR for the presence of nuc and coa virulence genes. The positive coa bands were subjected to restriction fragment length polymorphism analysis (RFLP) using AluI enzyme and sequenced. The results confirmed 10 isolates as Staphylococcus aureus. All ten isolates harbored nuc and coa genes. The RFLP analysis demonstrated five different patterns, and the genetic lineage of recovered isolates was related to a human origin strain sharing 75% homology. This alerts for the potential public health hazard of companion pets. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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