| Autor: |
Liu, Ying-ying, Liu, Xiao-kang, Cui, Xiao-die, Chen, Min, Li, Shuai-hua, He, Dan-dan, Liu, Jian-hua, Yuan, Li, Hu, Gong-zheng, Pan, Yu-shan |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 7/3/2020, Vol. 11, p1-8, 8p |
| Abstrakt: |
The objective of this study was to explore the genetic and biological features of the tet (M)-harboring plasmid pTS14 in Salmonella enterica strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability, growth kinetics, and starvation survival experiments. Strain S14, belonging to ST3007, harbored a 119-kb tet (M)-bearing IncF2:A1:B1 conjugative plasmid pTS14. The plasmid pTS14 contained a novel transposon Tn 6709 with the genetic structure IS 26 - tnpA1 - tnpA2 -Δ orf13 - LP - tet (M)- tnpX -Δ tnpR -IS 26 , and the resistance genes tet (B), tet (D), strAB , sul2 , and bla TEM–1b. In addition, pTS14 was found to be highly stable in the recipient strain E. coli J53. The transconjugant TS14 exhibited a higher survival ratio than E. coli J53 under permanent starvation-induced stress. The tet (M)-bearing IncF2 epidemic plasmid lineage may accelerate the dissemination of tet (M) and other genes by coselection, which could constitute a potentially serious threat to clinical treatment regimens. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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