| Autor: |
Phuong-Thao Nguyen-Thi, Quoc Phong Le, Stoldt, Volker R., Ngoc Quyen Tran, Anh Tho Le, Khon Huynh |
| Předmět: |
|
| Zdroj: |
Molecular & Cellular Biomechanics; 2020, Vol. 17 Issue 3, p113-118, 6p |
| Abstrakt: |
Fibrillar fibronectin (FFN) is a biological active form of FN which form linear and branched meshwork around cells and support cellular activities. Previous studies have demonstrated that shear stress can induce cell-free FN fibrillogenesis. In this study, we further examined the effect of shear stress conditions on morphology of formed FFN and preliminarily looked for relationship between FFN's morphology and cell adhesion. Plasma FN at 50 µg/ml was perfused through channel slides at shear rates of 500 s-1 or 4000 s-1. Our results showed that there were four FFN structures formed: (1) FN nodules, (2) fibril in different sizes (3) with or without nodule attachment, and (4) fibrillar matrix. At 4000 s-1, FFN fibrils was formed within the first 10 min and reached the highest surface coverage only after 20 min. In contrast, FFN formation was significant more slowly at 500 s-1 at which only FN nodules and small fibrils were formed. Platelets bound on thin layer of FN and rarely found on large FN fibrils. In contrast, fibroblast stretched their shape on platform of FFN matrix and bound actively to all types of FFNs. Taken together, our data suggests a morphological dependent biological activity of FFN. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
