| Abstrakt: |
Obesity is a potential source of various chronic diseases such as type-2 diabetes, hypertension and hyperlipidemia. This study aimed to investigate the effects of Jinchang- mi (JCM) and its synergistic effect with Garcinia gummi-gutta (GG) on highfat- diet (HFD)-fed obese mice. Four-week-old male C57BL/6J mice (n=36) were divided into 6 groups viz. Normal, HFD, JCM320, JCM320 + GG150, JCM640 + GG150 and GG150). The mice of all the groups were fed HFD for 3 weeks to induce obesity, followed by oral administration of different treatments daily for 7 weeks along with HFD. The mice were euthanized 24 h after the final administration of different treatments. Blood, liver, and white adipose tissue samples were collected from each mouse for further analysis including serum chemistry, hematoxylin and eosin staining, and real-time PCR. The administration of JCM, GG, or their combination partially inhibited body weight gain and feed efficiency; and reduced the levels of serum glucose and glutamic pyruvic transaminase. The accumulation of white adipose tissue was significantly inhibited in the GG150 group. There were no significant differences in the expression levels of genes related to the browning of white adipose tissue among different treatment groups. Liver size and weight were markedly increased by the HFD group while mice restored the liver size and weight following sample treatment (especially, the JCM320 and JCM320 + GG150 groups). In conclusion, oral administration of JCM with GG exhibited slight anti-obesity effects by reducing body weight gain (accumulation of adipose tissue) and suppressing the progression of hepatic steatosis by attenuating micro- and macrovesicular lipid accumulation. [ABSTRACT FROM AUTHOR] |
