| Autor: |
PAULINO-GONZALEZ, ANGEL DAVID, HIROSHI SAKAGAMI, KENJIRO BANDOW, KANDA, Y. UMIKO, YUKO NAGASAWA, YASUSHI HIBINO, HIROSHI NAKAJIMA, SATOSHI YOKOSE, OSAMU AMANO, NAKAYA, GIICHIROU, YUKARI KOGA-OGAWA, AKIYOSHI SHIROTO, NOBESAWA, T. ADAMASA, DAISUKE UEDA, SACHIE NAKATANI, KENJI KOBATA, YOSUKE IIJIMA, SHINSUKE IFUKU, MASAJI YAMAMOTO, GARCIA-CONTRERAS, RENE |
| Předmět: |
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| Zdroj: |
In Vivo; Jul/Aug2020, Vol. 34 Issue 4, p1729-1738, 10p |
| Abstrakt: |
Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: Chi-N P was prepared by mixing magnetic NP with chitosan FL-80. P article size was determined by scanning and transmissione lectron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs bycombination index, PGE2 production in human gingivalfibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE2 production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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