| Abstrakt: |
Objective: To explore the active ingredients and potential mechanism of Aidi injection in the treatment of hepatocellular carcinoma by network pharmacology. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Traditional Chinese Medicines Integrated Database (TCMID) were used to screen the active ingredients of four traditional Chinese medicines of Renshen, Huangqi, Ciwujia, and Banmao and corresponding potential targets. Screening of hepatocellular carcinoma-related targets through the Online Mendelian Inheritance in Man (OMIM) and GeneCards Suite (The Human Gene Database) database platforms. The drug and disease targets are merged to obtain the intersection, and the information is imported into Cytoscape 3.7.2 to construct a network diagram of the active ingredients of Aidi injection and related targets of hepatocellular carcinoma, and the topology analysis is performed. A protein-protein interaction (PPI) network was constructed and analyzed using the STRING online analysis platform. Uses the Database for Annotation, Visualization and Integrated Discovery (DAVID) to perform GO function enrichment analysis of targets and enrichment of KEGG pathways analysis. Results: A total of 33 potential active ingredients were screened from Aidi injection for treating hepatocellular carcinoma, including quercetin, kaempferol, beta-sitosterol, isorhamnetin and other important active ingredients. There are 106 potential targets for active ingredient action, 6,677 diseaserelated targets, and 89 drug-disease common targets. Through the network diagram, it was found that the highest degree of target is PTGS1.In the PPI graph, a total of 87 nodes. Among them, the higher degree values include IL6, CASP3, VEGFA, MAPK8, JUN, EGFR, MYC, PTGS2 and FOS.A total of 60 related signal pathways were obtained by GO enrichment analysis. It mainly involves biological processes such as inhibiting abnormal proliferation and differentiation of hepatocellular carcinoma cells, inhibiting angiogenesis of hepatocellular carcinoma, regulating cell cycle and promoting apoptosis. KEGG pathway enrichment analysis screened a total of eight significantly different signal pathways. Among them, P53, VEGF, MAPK, Toll-like receptor, ErbB signaling pathways play an important role in treatment. Conclusion: This study initially revealed the potential mechanism of multi-component, multi-target and multi-pathway treatment of Aidi injection for hepatocellular carcinoma, and provided ideas for the subsequent verification of the molecular mechanism of Aidi injection for hepatocellular carcinoma. [ABSTRACT FROM AUTHOR] |
