Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract.

Autor:	Kim, Choon Young, Yu, Qi-Ming, Kong, Hyun-Joo, Lee, Joo-Yeon, Yang, Kyung-Mi, Seo, Jung-Sook
Předmět:	REACTIVE oxygen species CYTOKINES DOSE-effect relationship in pharmacology FLAVONOIDS GENE expression HIGH performance liquid chromatography INFLAMMATION INFLAMMATORY mediators LIVER cells PHENOLS QUERCETIN FUNCTIONAL foods PHYTOCHEMICALS PLANT extracts OXIDATIVE stress AGRIMONY GENISTEIN LIPOPOLYSACCHARIDES GLUTATHIONE peroxidase FLAVONES PHARMACODYNAMICS
Zdroj:	Evidence-based Complementary & Alternative Medicine (eCAM); 6/16/2020, p1-10, 10p, 2 Charts, 5 Graphs
Abstrakt:	The purpose of this study is to investigate the effect of Agrimonia pilosa Ledeb. extract (APLE) on lipopolysaccharide- (LPS-) induced cell damage in hepatocytes with a focus on antioxidant and anti-inflammatory activities. Total antioxidant and anti-inflammatory activities of APLE itself were analyzed and phytochemical analysis was performed. Moreover, inhibitory effects of APLE on LPS-induced oxidative stress and inflammation were assessed in human HepG2 hepatocytes. APLE was found to exert α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and nitrite scavenging activities and reducing power in a dose-dependent manner. The total phenolic and flavonoid contents of APLE were 44.30 ± 1.61 mg GAE/g and 29.65 ± 1.81 mg QE/g, respectively. HPLC analysis revealed that gallic acid is the major phenolic compound in APLE, followed by rutin, genistein, taxifolin, quercetin, luteolin, and apigenin, in descending order. Treatment of 100 and 200 μg/mL APLE significantly reduced LPS-stimulated intracellular reactive oxygen species production to the basal level without any cytotoxicity. Oppositely, APLE reversed LPS-suppressed expression of glutathione peroxidase gene and protein. Consistent with this result, APLE suppressed LPS-triggered expression of proinflammatory cytokine genes in a dose-dependent manner. These results reinforce the fact that the antioxidant and anti-inflammatory activity of APLE helps protect hepatocytes from LPS. Thus, APLE may be utilized as a bioactive ingredient in functional foods. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text