| Autor: |
Elkoumi, Mohamed A, Allah, Mayy AN, Mohamed, Faisal Y, Boraey, Naglaa F, Abdellatif, Sawsan H, Shehab, Mohamed MM, Sherif, Ahmed H, Akeel, Nagwa E, Saleh, Rabab M, Elshreif, Anas M, Abdelrahman, Hind M, Soliman, Attia A, Emam, Ahmed A, Youssef, Manal AA, Fahmy, Dalia S, Sallam, Mohammad M, Nawara, Abdalla M, Elgohary, Elsayed A, Ismael, Ali, El-Kaffas, Sameh MH |
| Zdroj: |
Lupus; Jun2020, Vol. 29 Issue 7, p767-775, 9p, 5 Charts |
| Abstrakt: |
Background: Recently, the interleukin-17A (IL-17A) gene has emerged as a potential candidate gene for autoimmune disorders, including systemic lupus erythematosus (SLE). Objectives: This study aimed to investigate whether IL-17A polymorphisms at rs2275913 G/A, rs8193036 C/T and rs3748067 C/T could be susceptibility markers for juvenile-onset SLE (JSLE) and lupus nephritis (LN) in Egyptian children and adolescents. Methods: In this multi-centre study, we genotyped 320 patients diagnosed with JSLE and 320 matched control children for three IL-17A polymorphisms at rs2275913 G/A, rs8193036 C/T and rs3748067 C/T using TaqMan probe-based real-time polymerase chain reaction. Meanwhile, IL-17A serum levels were assessed using ELISA. Results: The IL-17 rs2275913 A/A genotype and A allele were more represented in JSLE patients compared to the control group (21% vs. 7%, odds ratio (OR) = 3.8, 95% confidence interval (CI) 1.78–5.5, p = 0.001, p Bonf = 0.003 for the A/A genotype; 37% vs. 29%, OR = 1.4, 95% CI 1.11–1.8, p = 0.003, p Bonf = 0.009 for the A allele. No significant difference was found for IL-17 rs8193036 and rs3748067 single nucleotide polymorphisms (SNPs) in genotype distribution or allele frequencies (p >0.05). Patients carrying the IL-17 rs2275913 A/A genotype and A allele were more likely to develop LN (OR = 5.64, 95% CI 2.39–13.77, p Bonf = 0.001 for the A/A genotype; OR = 2.73, 95% CI 1.84–4.07, p Bonf = 0.02 for the A allele). Conclusion: The IL-17 rs2275913 A allele and A/A genotype were associated with high IL-17 serum levels and may contribute to susceptibility to JSLE and the development of LN in Egyptian children and adolescents. However, no significant association was evident between the studied IL-17A SNPs and other clinical phenotypes, disease activity scores or laboratory profile of JSLE. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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