| Autor: |
Johansson, A.-K., Sjöstrand, M., Tomaki, M., Samulesson, A.-M., Lötvall, J. |
| Předmět: |
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| Zdroj: |
Allergy; Oct2004, Vol. 59 Issue 10, p1080-1086, 7p |
| Abstrakt: |
The specific mechanisms that alter bone marrow (BM) eosinophilopoiesis in allergen-induced inflammation are poorly understood. The aims of this study were to evaluate (a) whether the number of BM CD34+ cells is altered due to allergen sensitization and exposurein vivoand (b) whether BM CD34+ cells produce and release interleukin (IL)-5, IL-3 and granulocyte macrophage-colony stimulating factor (GM-CSF) after stimulationin vitro. A mouse model of ovalbumin (OVA)-induced airway inflammation was used. Bone marrow CD34+ cells were culturedin vitroand the cytokine release was measured by enzyme-linked immunosorbent assay. The IL-5-production from CD34+ cells was confirmed by immunocytochemistry. Airway allergen exposure increased the number of BM CD34+ cells (P = 0.01). Bone marrow CD34+ cells produced IL-5 when stimulated with the allergen OVAin vitro, but not IL-3 or GM-CSF. Nonspecific stimulus with calcium ionophore and phorbol-myristate-acetate of BM CD34+ cells caused release of IL-5, IL-3 and GM-CSF. The induced release of IL-5 was increased in alum-injectedvsnaive mice (P = 0.02), but was not affected by allergen sensitization and exposure. The release of IL-3 and GM-CSF was increased after allergen sensitization and exposure (P < 0.02). In conclusion, allergen can stimulate BM CD34+ cells to produce IL-5 protein. It is likely that the CD34+ cells have autocrine functions and thereby regulate the early stages of BM eosinophilopoiesis induced by airway allergen exposure. Alum, a commonly used adjuvant, enhances the release of IL-5 and may thereby enhance eosinophilopoiesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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