| Abstrakt: |
Background: cholestasis is a prevalent health problem associated with liver oxidative stress, inflammation, and fibrosis. Quercetin has been shown to afford a beneficial effect in a variety of liver diseases. Aim: This study was designed to investigate the potential protective effect of quercetin on liver cholestasis and the possible underlying mechanisms in a rat model of bile duct ligation (BDL). Design: Experimental study. Methods: This study was carried out on adult male Wister rats which were randomly divided into: Sham, BDL and BDL- quercetin treated (BDL- Q) groups. Quercetin was given by gavage in a dose of 50 mg/kg/day. Results: Bile duct ligation resulted in a significant increase in serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and liver levels of myeloperoxidase (MPO), tumor necrosis factor alpha (TNFa), and transforming growth factor beta 1(TGF-b1), along with a significant decrease in serum levels of total proteins (TP) and liver glutathione peroxidase(GPX) in BDL group versus sham group. Quercetin treatment significantly lowered serum levels of AST, ALT, ALP, and MPO, TNF-a, and TGF-b1 in liver tissues associated with a significant increase in serum TP and liver GPX in BDL-Q group versus BDL rats. Histological studies revealed enhancement of inflammation and a significant increase in the percentage area of collagen deposition in BDL versus sham group. These changes were attenuated in BDL-Q group compared to BDL rats. Conclusions: Quercetin alleviated cholestasis induced liver injury and improved liver function possibly via attenuating liver oxidative stress, inflammation and fibrosis. [ABSTRACT FROM AUTHOR] |
