Bacterial contamination rate of platelet components by primary culture: a systematic review and meta-analysis.

Autor:	White, Sandra K., Schmidt, Robert L., Walker, Brandon S., Metcalf, Ryan A.
Předmět:	BACTERIAL contamination META-analysis BLOOD platelets CULTURE BACTERIAL disease transmission PLATELET-rich plasma RESEARCH CELL culture BACTERIOLOGY technique RESEARCH methodology SYSTEMATIC reviews BLOOD transfusion reaction EVALUATION research MEDICAL cooperation COMPARATIVE studies HEMAPHERESIS BLOOD platelet transfusion BACTERIAL diseases DRUG adulteration RED blood cell transfusion
Zdroj:	Transfusion; May2020, Vol. 60 Issue 5, p986-996, 11p, 2 Diagrams, 5 Charts, 1 Graph
Abstrakt:	Background: Platelets have the highest bacterial contamination risk of all blood components, and septic transfusion reactions remain a problem. A good estimate of contamination rates could provide information about residual risk and inform optimal testing strategies. We performed a systematic review and meta-analysis of platelet contamination rates by primary culture.Study Design and Methods: A literature search in December 2019 identified articles on platelet contamination rates using primary culture. We used meta-analysis to estimate the overall rate of contamination and meta-regression to identify heterogeneity. We studied the following sources of heterogeneity: collection method, sample volume, positivity criteria, and study date. Contamination rate estimates were obtained for apheresis (AP), platelet rich plasma (PRP), and buffy coat (BC) collection methods.Results: The search identified 6102 studies, and 22 were included for meta-analysis. Among these 22 studies, there were 21 AP cohorts (4,072,022 components), 4 PRP cohorts (138,869 components), and 15 BC cohorts (1,474,679 components). The overall mean contamination rate per 1000 components was 0.51 (95% CI: 0.38-0.67) including AP (0.23, 95% CI: 0.18-0.28), PRP, (0.38, 95% CI: 0.15-0.70), and BC (1.12, 95% CI: 0.51-1.96). There was considerable variability within each collection method. Sample volume, positivity criteria, and publication year were significant sources of heterogeneity.Conclusion: The bacterial contamination rate of platelets by primary culture is 1 in 1961. AP and PRP components showed a lower contamination rate than BC components. There is clinically significant between-study variability for each method. Larger sample volumes increased sensitivity, and bacterial contamination rates have decreased over time. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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