| Autor: |
Savas, Elif, Karaagac, Oznur, Kockar, Hakan, Kaya, Mihrap Yaşar, Türkoğlu, Sümeyye Aydoğan, Kockar, Feray |
| Předmět: |
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| Zdroj: |
Sensors & Materials; 2020, Vol. 32 Issue 2, Part 3, p799-808, 10p |
| Abstrakt: |
The aim of this study is to develop stable superparamagnetic iron oxide nanoparticles (SPIONs) for the immobilization of Îβ-glucosidase. The goal is to figure out the possibility of applying immobilization techniques in the debittering of olives. In the present study, SPIONs with a size of ~9 nm were synthesized by co-precipitation, and immobilization was carried out by the formation of covalent bonds between Îβ-glucosidase and SPIONs. The properties of the free enzymes immobilized with SPIONs were compared by using commercial Îβ-glucosidase as a standard. The immobilized enzymes were characterized by using techniques including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The results showed that SPIONs are an effective and suitable material for the immobilization of Îβ-glucosidase as well as a potential nanomaterial for the elimination of olive bitterness originating from oleuropein. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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