| Autor: |
Oshiki, Mamoru, Yamada, Koshiro, Kato, Itsuki, Okoshi, Kento, Imai, Toshiro, Yamaguchi, Takashi, Araki, Nobuo |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; Jun2020, Vol. 42 Issue 6, p979-985, 7p |
| Abstrakt: |
Objectives: To synthesize hydrazine (N2H4) from ammonium and hydroxylamine (NH2OH) using an anaerobic ammonium oxidation (anammox) bacterium, Candidatus Kuenenia stuttgartiensis. Results: K. stuttgartiensis cells were anoxically cultivated with the addition of ammonium (2 mM) and NH2OH (1–100 mM) at pH 6–10.5, and 4–65 °C to examine the favorable cultivation conditions for N2H4 production. The influence of NH2OH concentration was more prominent than that of pH and temperature, and NH2OH concentration higher than 1 mM deteriorated N2H4 yields significantly. The following conditions were found to be favorable for N2H4 production using K. stuttgartiensis cells: pH 9, 38 °C, and < 1 mM NH2OH. In a continuous-feed system operated at these conditions, K. stuttgartiensis cells produced N2H4 with a maximum concentration of 0.65 mM, which is the highest N2H4 concentration previously reported in biological processes. Conclusions: Optimal cultivation conditions for K. stuttgartiensis for N2H4 production were successfully determined, and the present study is the first to document potential biological N2H4 production using anammox bacteria. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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