| Autor: |
Le, Thanh Thi Minh, Hoang, Anh Thi Hong, Nguyen, Nhue Phuong, Le, Thuy Thi Bich, Trinh, Ha Thi Thu, Vo, Thuy Thi Bich, Quyen, Dong Van |
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| Zdroj: |
Biotechnology Letters; Jun2020, Vol. 42 Issue 6, p987-995, 9p |
| Abstrakt: |
The isolation, identification and characterization of a novel huperzine A (HupA)-producing fungal strain Rsp5.2 isolated from Huperzia serrata (Thunb. ex Murray) Trev. in Vietnam. The fifty-eight endophytic fungi were recovered from roots of natural H. serrata in Lao Cai province of northern Vietnam and screened for HupA-producing by thin-layer chromatography (TLC). The only one of the 58 strains, Rsp5.2, could produce HupA. The amount of HupA produced by Rsp5.2 was quantified to be 19.45 µg g−1 dried mycelium by high-performance liquid chromatography (HPLC). Acetylcholine esterase (AchE) inhibition (IC50) of the crude HupA extract from Rsp5.2 fermentation broth was 2.849 ± 0.0026 µg mL−1. The fungus was identified as Fusarium sp. Rsp5.2 by morphological characteristics and Internal Transcribed Spacer (ITS) sequences. This is the first report of Fusarium sp. as a HupA-producing endophyte isolated from H. serrata. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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