| Autor: |
Hopp, B., Smausz, T., Antal, Zs., Kresz, N., Bor, Zs., Chrisey, D. |
| Předmět: |
|
| Zdroj: |
Journal of Applied Physics; 9/15/2004, Vol. 96 Issue 6, p3478-3481, 4p, 1 Black and White Photograph, 1 Diagram, 1 Graph |
| Abstrakt: |
We present an investigation on absorbing film assisted laser induced forward transfer (AFA-LIFT) of fungus (Trichoderma) conidia. A KrF excimer laser beam [λ=248 nm,FWHM=30 ns (FWHM, full width at half maximum)] was directed through a quartz plate and focused onto its silver coated surface where conidia of the Trichoderma strain were uniformly spread. The laser fluence was varied in the range of 0–2600 mJ/cm2 and each laser pulse transferred a pixel of target material. The average irradiated area was 8×10-2 mm2. After the transfer procedure, the yeast extract medium covered glass slide and the transferred conidia patterns were incubated for 20 h and then observed using an optical microscope. The transferred conidia pixels were germinated and the areas of the culture medium surfaces covered by the pixels were evaluated as a function of laser fluence. As the laser fluence was increased from 0 to 355 mJ/cm2 the transferred and germinated pixel area increased from 0 to 0.25 mm2. Further increase in fluence resulted in a drastic decrease down to an approximately constant value of 0.06 mm2. The yield of successful transfer by AFA-LIFT and germination was as much as 75% at 355 mJ/cm2. The results prove that AFA-LIFT can successfully be applied for the controlled transfer of biological objects. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
