DNA damage induced during mitosis undergoes DNA repair synthesis.

Autor:	Gomez Godinez, Veronica, Kabbara, Sami, Sherman, Adria, Wu, Tao, Cohen, Shirli, Kong, Xiangduo, Maravillas-Montero, Jose Luis, Shi, Zhixia, Preece, Daryl, Yokomori, Kyoko, Berns, Michael W.
Předmět:	DNA synthesis DNA damage MITOSIS PREMATURE aging (Medicine) DNA mismatch repair LASER damage CHROMOSOMES DNA repair
Zdroj:	PLoS ONE; 4/28/2020, Vol. 15 Issue 4, p1-27, 27p
Abstrakt:	Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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