| Autor: |
Sattar, Rand Jabbar, Ali, Entesar Hussain |
| Předmět: |
|
| Zdroj: |
Plant Archives (09725210); 2019, Vol. 19 Issue 2, p4379-4385, 7p |
| Abstrakt: |
P. aeruginosa was isolated from burns patient to ensure that the isolate was belonging to P. aeruginosa various tests are made such as staining techniques, biochemical assay, morphological and sensitivity test. The gram stain and biochemical test result show rod pink gram negative bacteria and ensure that the isolate was belong to (P. aeruginosa). Optimization education for bacterial growth were done by used more than pH and temperature and it was found that the best conditions for the production and increase the number of bacteria at pH 5.5 with bacterial number (4.5×108 cells) and Temperature 37°C with bacterial number (5.25×108 cells). Extracellular enzyme was extracted by cool centrifuge 10000 × g for 20 min in 4°C, the cell pellet was re-used as a crude enzymatic extract and discard supernatant cell. Purification of Lipase was accomplished by using Salt precipitation, Ion exchange and gel filtrations chromatographic techniques. The result shows that, gel filtration has optimal specific activity and purification fold at (4U/ml), purification fold 5.7 times. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
