| Autor: |
Al-Swedi, Fadil G., Al-Juthery, Hayyawi W. A., Alawadi, Hussam F. Najeeb |
| Předmět: |
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| Zdroj: |
Plant Archives (09725210); 2019, Vol. 19 Issue 2, p2967-2972, 6p |
| Abstrakt: |
A study in vitro has been developed for efficient regeneration of shoots from Brassica oleracea. Using 4-day old cotyledons were used with petioles as explants and a combination of kinetin and Indol-Butyric Acid (IBA) in the regeneration media, after 2 weeks up to 80% of explants produced shoots in culture. a kinetin concentration of 2mgL-1 and IBA concentration of l mg -I were found in optimal conditions for regenerations. Regeneration potential had affected by light intensity. Rooting occurred simultaneously with shoot formation on these media and the resultant shoots could be rooted readily on minimal medium. This method would be widely applicable to Brassica oleracea cultivars where the genotype dependency was investigated and indicated. Histological studies after the initiation of meristematic activity in the cells indicated the development of multiple shoot from the petioles cut ends of the explants. Protocols involving cotyledonary petioles which described the compatible with previously reported Agrobacterium - mediated transformation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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