Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells.

Autor: Than, Uyen Thi Trang, Le, Huyen Thi, Hoang, Diem Huong, Nguyen, Xuan-Hung, Pham, Cuong Thi, Bui, Khanh Thi Van, Bui, Hue Thi Hong, Nguyen, Phong Van, Nguyen, Tu Dac, Do, Thu Thi Hoai, Chu, Thao Thi, Bui, Anh Viet, Nguyen, Liem Thanh, Hoang, Nhung Thi My
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Zdroj: International Journal of Molecular Sciences; Mar2020, Vol. 21 Issue 5, p1834, 1p
Abstrakt: (1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy—e.g., vaccines—for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation of allogeneic DCs and their exosomes will be material for vaccinating against cancer. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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