| Autor: |
Mukherjee, Somnath, Erramilli, Satchal K., Ammirati, Mark, Alvarez, Frances J. D., Fennell, Kimberly F., Purdy, Michael D., Skrobek, Blazej M., Radziwon, Katarzyna, Coukos, John, Kang, Yanyong, Dutka, Przemysław, Gao, Xiang, Qiu, Xiayang, Yeager, Mark, Eric Xu, H., Han, Seungil, Kossiakoff, Anthony A. |
| Předmět: |
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| Zdroj: |
Nature Communications; 3/27/2020, Vol. 11 Issue 1, p1-14, 14p |
| Abstrakt: |
We propose the concept of universal fiducials based on a set of pre-made semi-synthetic antibodies (sABs) generated by customized phage display selections against the fusion protein BRIL, an engineered variant of apocytochrome b562a. These sABs can bind to BRIL fused either into the loops or termini of different GPCRs, ion channels, receptors and transporters without disrupting their structure. A crystal structure of BRIL in complex with an affinity-matured sAB (BAG2) that bound to all systems tested delineates the footprint of interaction. Negative stain and cryoEM data of several examples of BRIL-membrane protein chimera highlight the effectiveness of the sABs as universal fiducial marks. Taken together with a cryoEM structure of sAB bound human nicotinic acetylcholine receptor, this work demonstrates that these anti-BRIL sABs can greatly enhance the particle properties leading to improved cryoEM outcomes, especially for challenging membrane proteins. Single particle cryo-electron microscopy of membrane proteins is limited by their small size and difficulty to orient. Here the authors generate recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure determination of BRIL fused membrane proteins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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