| Autor: |
Narayanan, J. Shankara, Slaughter, Gymama |
| Zdroj: |
IEEE Sensors Journal; 4/15/2020, Vol. 20 Issue 8, p4034-4040, 7p |
| Abstrakt: |
Lactate, a key metabolite of the anerobic metabolic pathway serves as a biomarker for lactic acidosis in clinical diagnostics. A variety of electrochemical biosensors have been exploited for the determination of lactate. Here we describe an improved electrochemical lactic acid biosensor that utilizes self-assembled monolayers (SAMs) of cysteamine-modified gold nanoparticles (AuNPs) on flexible tungsten microwire electrode to immobilize lactate dehydrogenase (LDH) to the microwire electrode. The fabricated AuNP-cysteamine-LDH electrode showed excellent behavior towards lactic acid at pH 6 and at 35 °C at an applied potential of +0.4 V (vs Ag/AgCl reference electrode). The biosensor exhibited a linear dynamic range of $500~\mu \text{M}$ to 7 mM with a very high sensitivity of $31.40~\mu \text{A}$ /mM cm2. The biosensor demonstrated good selectivity towards lactic acid, wherein a nafion layer was utilized to screen out interfering species. The AuNP-cysteamine-LDH electrode remained stable up to 18 days. The practicality of the AuNP-cysteamine-LDH electrode was assessed by determining lactic acid concentrations in sera and a sensitivity of $73.16~\mu \text{A}$ / mM cm2 was observed up to 5 mM. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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