| Autor: |
Bouts, A. H. M., Davin, J. C., Krediet, R. T., Monnens, L. A. H., Nauta, J., Schrodertt, C. H., Van Liert, R. A. W., Out, T. A. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Sep2004, Vol. 137 Issue 3, p589-594, 6p |
| Abstrakt: |
Reduced serum IgG and subclass levels have been demonstrated in children with chronic renal failure. To study possible causes of this reduction, we analysed B cell subset composition, T helper cell frequencies and immunoglobulin (Ig) production capacity in vitro in children with chronic renal failure, with or without dialysis treatment. B cell subsets were characterized by determining CD27, IgM, IgD and CD5 expression within the CD19+ population. Intracellular expression of interferon (IFN)- γ, interleukin (IL)-2 and IL-4 in PMA/ionomycin-stimulated peripheral blood mononuclear cells (PBMC) was used to evaluate T helper frequencies. The capacity of B cells to secrete Ig in vitro was determined by measuring IgG1, IgG2 and IgM in culture supernatants of anti-CD2/CD28 monoclonal antibody (MoAb)- or SAC/IL-2-stimulated PBMC. Memory B cell numbers (identified as percentage or absolute number of CD19+ IgM¯IgD¯ or CD19+CD27+ lymphocytes) were lower in children treated with haemodialysis (HD), peritoneal dialysis (PD) and children with chronic renal failure before starting dialysis treatment (CRF) compared to healthy controls (HC) ( P < 0·05). Compared with HC, CD5+ (naive) B cells were reduced in HD-treated patients but not for PD or for children with chronic renal failure before starting dialysis treatment (CRF). No significant differences in CD4+ T helper cell subsets were found between the groups. However, CRF children had a higher percentage of IFN- γ producing CD8+ T lymphocytes compared to HC ( P = 0·02). Finally, IgG1, IgG2 and IgM production in vitro was similar in the four groups. In conclusion, significantly lower numbers of memory type B cells were found in children with chronic renal failure compared to healthy controls. This reduction may contribute to the low Ig levels found in these children. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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