The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles.

Autor: Matorras, Roberto, Martinez-Arranz, Ibon, Arretxe, Enara, Iruarrizaga-Lejarreta, Marta, Corral, Blanca, Ibañez-Perez, Jone, Exposito, Antonia, Prieto, Begoña, Elortza, Felix, Alonso, Cristina
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Zdroj: Journal of Assisted Reproduction & Genetics; Feb2020, Vol. 37 Issue 2, p385-394, 10p
Abstrakt: Objective: To characterize the most relevant changes in the lipidome of endometrial fluid aspirate (EFA) in non-implantative cycles. Design: Lipidomics in a prospective cohort study. Settings: Reproductive unit of a university hospital. Patients: Twenty-nine women undergoing an IVF cycle. Fifteen achieved pregnancy and 14 did not. Intervention: Endometrial fluid aspiration immediately before performing embryo transfer. Main outcome measures: Clinical pregnancy rate and lipidomic profiles obtained on an ultra-high performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-ToF-MS)-based analytical platform. Results: The comparative analysis of the lipidomic patterns of endometrial fluid in implantative and non-implantative IVF cycles revealed eight altered metabolites: seven glycerophospholipids and an omega-6 polyunsaturated fatty acid. Then, women with a non-implantative cycle were accurately classified with a support vector machine algorithm including these eight lipid metabolites. The diagnostic performances of the algorithm showed an area under the receiver operating characteristic curve, sensitivity, specificity, and accuracy of 0.893 ± 0.07, 85.7%, 80.0%, and 82.8%, respectively. Conclusion: A predictive lipidomic signature linked to the implantative status of the endometrial fluid has been found. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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