| Abstrakt: |
Black-Bone Silky Fowl is a kind of chicken with medicinal value, the active peptides with high nutritional value can be obtained by enzymatic hydrolysis of the muscle protein. The separation and purification of active peptides are the basis of the application value of Black-Bone Silky Fowl. Two common methods, C18 reversed-phase chromatography and TSKgel filtration chromatography, were used to optimize isolation conditions for active peptides from Black-Bone Silky Fowl based on HPLC-PDA. The effects of these aspects on the isolation were studied, such as a raw material mixture ratio of mobile phase, flow rate, injection amount and wavelength. Isolation effects were based on the peak numbers in combination with peak resolution. When isolated by C18 reversed-phase chromatography, the number of peaks was increased by the decrease of methanol concentration and flow rate. Moreover, peak numbers were almost the same but not peak resolution. The optimizing isolation conditions were pH 6. 0 sodium phosphate/6% methanol/distilled water and 0.2 mL/min flow rate. When isolated by TSKgel filtration chromatography, isolation effect of acetonitrile was better than methanol, and gradient elution effect of acetonitrile was better. The optimizing isolation conditions were 0. 1% trifluoroacetic acid/( 10%-25%) acetonitrile/distilled water and 0. 5 mL/min flow rate. [ABSTRACT FROM AUTHOR] |
