| Autor: |
Hatalová, E., Bednárová, V., Majlingová, S., Guman, T., Pápajová, I., Halánová, M., Juriš, P. |
| Zdroj: |
Slovak Veterinary Journal / Slovenský Veterinársky časopis; 2019, Vol. 44 Issue 2, p157-159, 3p |
| Abstrakt: |
This study was focused on the diagnostic of intestinal protozoa in children suffering from diarrhea. A total of 48 samples were obtained and divided into two groups: group A (24 samples from children hospitalized at the Clinic of pediatric gastroenterology (Children`s faculty hospital in Košice) and group B (24 samples from children living in low hygienic standards in Košice`s Luník IX residential district). All samples were tested for the presence of enteric protozoa by a commercial ELISA kit and two PCR analyses using primers targeting the GP60 gene of C. parvum and C. hominis, and primers targeting the SSU rDNA of intestinal protozoa. ELISA confirmed the presence of Cryptosporidum spp. in two samples, although PCR analysis targeting the GP60 gene excluded the presence of C. parvum or C. hominis. After using the primers targeting the SSU rDNA of intestinal protozoa, 24 samples were positive. This can be explained by the presence of different species of Cryptosporidium in samples, or by the presence of other pathogens (phylum Apicomplexa, gregarines, Neoba lantidium coli), although further analyses are needed to confirm this possibility. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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