| Autor: |
Yangyi MAO, Huidi LUO, Zhiwu WANG, Peng ZHAO, Guanwu ZHANG, Jun LI, Hongyu GUO, Li ZHANG, Lina XUE, Wenqing DANG |
| Předmět: |
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| Zdroj: |
Agricultural Biotechnology (2164-4993); Aug2019, Vol. 8 Issue 4, p92-95, 4p |
| Abstrakt: |
This study was conducted to investigate the effects of different concentrations of β-mercaptoetlianol on the development al)ility of oocytes in Dorper sheep at the age of 6 weeks. In this study, 6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin, and A and B grade cumulus OOGyte complexes were obtained (COCs) with different concentrations of (0, 50, 70 and 100 μmol/L) of β-mercaptoethanol in the mature liquid. The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated. The results showed that compared with the cleavage rate (59.13% ) and the blastocyst rate (12. 17%) of the control group, the cleavage rales (60.87%, 63.48% and 65.22% ) and blastocyst rates (14.78%, 17.39% and 21.74%) of the test groups 1, 11 and 111 were higher (P >0.05), and the order was test group 111 > test group 11 > test group 1 > control group. The cleavage rate and blastocyst rate of the 100 μmol/L β-mercaptoelhanol group increased by 6. 09 percentage points and 9.57 percentage points, respectively. It indicated that the addition of a certain amount of p-mereaptoelhanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep. When the β-mercaptoethanol concentration was within 100 μmol/L, the cleavage rate and the blastocyst rate of oocytes were on the rise. The optimal concentration of β-mercaptoethanol in the mature liquid still needs further study. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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