| Autor: |
Grover, Dhruv, Katsuki, Takeo, Li, Jinfang, Dawkins, Thomas J., Greenspan, Ralph J. |
| Předmět: |
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| Zdroj: |
Nature Communications; 1/30/2020, Vol. 11 Issue 1, p1-10, 10p |
| Abstrakt: |
Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating. In vivo recordings of free-moving Drosophila neural activity are limited to clearly-separated flies in a stable focal plane. Here the authors improved on their Flyception imaging system to remove these constraints, and image neural activity in the male fly brain during various stages of the mating sequence. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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