Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484.

Autor:	Xia, Changhong, Zhang, Dongsheng, Li, Yanmei, Chen, Jie, Zhou, Haibo, Nie, Long, Sun, Yanyan, Guo, Siyan, Cao, Jianbiao, Zhou, Fangzheng, Li, Junlai
Předmět:	CELL proliferation ANTINEOPLASTIC agents CELL lines GENE expression HEPATOCELLULAR carcinoma PEPTIDES POLYMERASE chain reaction REVERSE transcriptase polymerase chain reaction CELL migration inhibition MATRIX metalloproteinases CHEMICAL inhibitors PHARMACODYNAMICS
Zdroj:	Journal of Research in Medical Sciences; Dec2019, Vol. 24 Issue 1, p26-26, 1p
Abstrakt:	Background: The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells. Materials and Methods: Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR. Results: With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed. Conclusion: TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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