| Abstrakt: |
Across sectional study was carried out to investigate the prevalence of Theileriosis in the local cows in Baghdad - Iraq during the period from March to May 2017. The study included 126 blood samples of the local cows of both sexes which were selected randomly. Thirty samples were excluded because they are suspected to be infected with others parasites according to microscopic test. The rest numbers of samples (96; 56 females and 40 males) were examined by microscopic and real-time polymerase chain reaction (qPCR).The results of microscopic examination revealed that the prevalence of Theileria annulata was 33.33%,where as the result of PCR technique was 57.29%.The results of comparison showed that however, the microscopic test have a high specificity (100%), but the sensitivity was too low (58.18%) andthe degree of agreement (Kappa=0.54) was a weak. The differences between the prevalence according to the two methods was significant (P<0.0001), therefore, the microscopic test is not efficient for detection of theilieria in the Iraqi local cows. Results also, showed that the effects of the status, age, sex, region, and month of data collection on some hematological parameters were significant (P<0.05). The means of MCH (21.51 pg) and MCHC (43.35 g/dL) in the infected animals were significantly (P<0.05) higher than the corresponding means (17.98 pg and 36.40 g/dL) in the non-infected. [ABSTRACT FROM AUTHOR] |
