| Abstrakt: |
This study were isolated and identified locally in the province of Thi-Qar, Iraq. These strains have shown their ability to produce of Enzyme Endo-β-1, 4-glucanase was determined after growing on carboxymethyl cellulose CMC medium. The ability of isolates was detected in the cellulose analysis after using the stain of Congo red and NaCl solution.The isolates were identified using Morphological, biochemical testsas VITEK2 and Molecular biology 16S rRNA tests.The gene sequence was compared with data available Gen Bank, NCBI data showed that they were new strains of B. subtilis strain M3 (MF455196), B. subtilis strain M4 (MF480418), B. subtilis strain M5(MF480424) and B. subtilis strain M6(MF480420) bacteria. Isolates were recorded in NCBI GenBank and were design for each evolutionary tree isolation. Endo-β-1, 4-glucanase was produced and the culture conditions like temperature, pH, and Incubation time,Incubator Shake and medium components like Carbon sources, nitrogen sources and role of natural substrates were optimized. Enzyme activity was measured using of dinitro salicylic acid DNS detector and spectrophotometer at 540 nm. [ABSTRACT FROM AUTHOR] |
