| Autor: |
Ghany, Soad M. Abdel, Ali, Esraa M. A., Ahmed, Amr E., Hozayen, Walaa G., Mohamed-Hussein, Aliae A. R., Elnaggar, Maha Salah, Hetta, Helal F. |
| Předmět: |
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| Zdroj: |
Middle East Journal of Cancer; Jan2020, Vol. 11 Issue 1, p50-58, 9p, 2 Charts, 6 Graphs |
| Abstrakt: |
Background: Detecting non-small-cell lung cancer at an early stage has become a great challenge due to the lack of a specific non-invasive marker. MicroRNAs are small, non-coding RNA molecules that play a role in carcinogenesis and cancer progression, as indicated by their abnormal expression in the patients' plasma. Herein, we investigated the plasma level of circulating miRNA-30a and miRNA-221 as noninvasive markers for an early detection of non-small-cell lung cancer. Method: A cross-sectional study was conducted at Assiut University Hospital, Egypt, to investigate miRNA-30a and miRNA-221 expression via quantitative realtime PCR in the plasma of patients with non-small-cell lung cancer (n=70) and healthy controls (n=34). Receiver operating curves were used to evaluate the diagnostic value of miRNA-221 and miRNA-30a in non-small-cell lung cancer. The relationship between both markers and patient clinical parameters was further assessed. Result: Circulating plasma miRNA-30a and miRNA-221 levels were significantly higher in the non-small-cell lung cancer patients compared with those in the healthy controls (P<0.05). There was a significant difference regarding the plasma miRNA- 30a level among the three groups (the highest levels were recorded in adenocarcinoma, followed by large cell carcinoma and squamous cell carcinoma). ROC curve analysis of miRNA-30a and miRNA-221 showed that specificity and sensitivity were 60% and 80%, and 40% and 75%, respectively. Conclusion: miRNA-30a and miRNA-221 may be non-invasive biomarkers for early detection and screening or therapeutic targets in patients with NSCLC. Future studies are warranted regarding the use of biomarkers as therapeutic targets. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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