Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase.

Autor:	Evseenko, Vasily A., Svyatchenko, Svetlana V., Kolosova, Natalia P., Kovrizhkina, Valentina L., Marchenko, Vasiliy Y., Durymanov, Aleksander G., Goncharova, Natalia I., Ryzhikov, Alexander B.
Předmět:	INFLUENZA B virus INFLUENZA A virus INFLUENZA viruses INFLUENZA A virus H1N1 subtype BINDING site assay AVIAN influenza A virus INFLUENZA A virus H3N2 subtype COMPARATIVE studies
Zdroj:	Archives of Virology; Jan2020, Vol. 165 Issue 1, p201-206, 6p
Abstrakt:	Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT50) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT50 was observed with the seasonal human H3N2 virus (45.5 °C). The IT50 values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca2+ binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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