| Autor: |
Kar, Mousumi, Pillai, Sujit, Sharma, Gaurav, Deshmukh, Nitin, Krishna Singh, Kunwar Nagendra |
| Předmět: |
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| Zdroj: |
Manipal Journal of Pharmaceutical Sciences; Sep2019, Vol. 5 Issue 2, p47-49, 3p |
| Abstrakt: |
Atorvastatin and Ramipril were simultaneously estimated in pharmaceutical dosage forms by developing fast and precise RP-HPLC method. Chromatographic separations on C18 column (4.6mmx250mm, 5µm) were achieved using mixture of 0.1% OPA buffer (pH-3.0): Acetonitrile: Methanol, (45:50:05 v/v). pH adjustment of aqueous phase was done by using 0.5% Triethylamine. The peak response was monitored at 227 nm after injecting the sample into HPLC system at a flow rate of 1.0 ml/min. The calibration curve was linear over the range of Ramipril 10-30µg / ml and Atorvastatin 20-60µg / ml. Atorvastatin's average RT was 9.2826±0.0107 and Ramipril was 4.548±0.0366. Atorvastatin's percentage recovery value is 99.47% and Ramipril's 98.50% which confirms the excipients do not interfere in the formulation. Thus, the suggested HPLC technique can therefore be used for the simultaneous determination of these two drugs in pharmaceutical dosage forms due to speed, simplicity and high accuracy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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